In our lab, we are trying to understand the cellular mechanisms involved in the coordination of hippocampal local circuit inhibition. In particular, we want to understand how interneurons integrate synaptic inputs and produce spikes, how the firing rate and timing of different subtypes of interneurons are regulated via the activation of specific inhibitory mechanisms, and how these processes are modified by rewarding or harmful experiences.
To address these complex questions, we use a combination of electrophysiology (field potential, patch clamp, single unit, and sharp recordings) in vitro and in vivo, two-photon microscopy (dendritic Ca2+ imaging and neurotransmitter uncaging), optogenetics, molecular biology, immunohistochemistry, and neuroanatomy in transgenic mouse models.
Our laboratory is funded by the Natural Sciences and Engineering Research Council of Canada, Canadian Institutes of Health Research, Savoy Foundation and Canada Foundation for Innovation. We are part of the Centre of Neurophotonics and of the Axis of Cellular and Molecular Neuroscience at Centre de Recherche l'Institut universitaire en santé mentale de Québec (CRIUSMQ) in Québec-city.
Lisa Topolnik, Laval University, Canada
Marlene Bartos, University of Freiburg, Germany
Deadline for abstract submission: 10 Jan 2014
Deadline for full article submission: 01 Jun 2014
Tyan L, Chamberland S, Magnin E, Camiré O, Francavilla R, David LS, Deisseroth K, Topolnik L
J Neurosci. 2014 Mar 26;34(13):4534-47. doi: 10.1523/JNEUROSCI.3813-13.2014.
Dendritic inhibition provided by interneuron-specific cells controls the firing rate and timing of the hippocampal feedback inhibitory circuitry.
A novel form of burst-firing-induced long-term potentiation has been uncovered at inhibitory synapses onto CCK-positive interneurons.
Welcome to Linda David, a Postdoc who joined the lab to work on plasticity of inhibitory synapses, using optogenetic approaches.
Welcome to Xiao Luo, a MSc student from the University of Dundee, UK. During his PhD Xiao will develop a new optogenetic approach to study cortico-hippocampal interactions.
June 2-12, 2014
Summer Imaging School "Frontiers in Neurophotonics"
Our lab will demonstrate advanced two-photon Ca2+ imaging and optogenetics experiments.
October 25, 2013
Lisa Topolnik and Marlene Bartos launched the research topic NEW INSIGHTS INTO DENDRITIC INHIBITION in Frontiers in Synaptic Neuroscience. Article submissions now open!
June 9-14, 2013
Hippocampal Spring Conference in Taormina, Sicily
Session organized by Dr. Lisa Topolnik: Activity-dependent regulation of hippocampal inhibition; a new look at synaptic and dendritic plasticity.